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Trypsin/Lys-C Mix, Mass Spec Grade
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Trypsin/Lys-C Mix, Mass Spec Grade
Trypsin/Lys-C Mix, Mass Spec Grade
Trypsin/Lys-C Mix, Mass Spec Grade from Promega
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Citations:
(233)
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Trypsin/Lys-C Mix, Mass Spec Grade
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Description
Trypsin/Lys-C Mix, Mass Spec Grade, is a mixture of Trypsin Gold, Mass Spectrometry Grade, and rLys-C, Mass Spec Grade. The Trypsin/Lys-C Mix is designed to improve digestion of proteins or protein mixtures in solution
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Trypsin/Lys-C Mix, Mass Spec Grade
Company
Promega
Price
Supplier Page
View Company Product Page
Catalog Number
V5071
Quantity
20microg
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Promega
2800 Woods Hollow Rd.
Madison
,
Wisconsin
United States
Phone
: 800-356-9526
Fax: 800-356-1970
Company Profile
Website:
http://www.promega.com
Citations (233)
(81)
CpxA Phosphatase Inhibitor Activates CpxRA and Is a Potential Treatment for Uropathogenic Escherichia coli in a Murine Model of Infection
Microbiology Spectrum
April 27, 2022
Kate R. Fortney, Sara N. Smith, Julia J. van Rensburg, Julie A. Brothwell, Jessi J. Gardner, Barry P. Katz, Nagib Ahsan, Adam S. Duerfeldt, Harry L. T. Mobley, Stanley M. Spinola
M urea lysis buffer. Equal amounts of proteins (100 μg) were subjected for overnight trypsin/LysC (
V5071
;
Promega
) digestion. One microgram of bovine serum albumin (BSA) was added to each sample as an internal
(82)
Sex-Dependent Synaptic Remodeling of the Somatosensory Cortex in Mice With Prenatal Methadone Exposure
Advances in Drug and Alcohol Research
April 25, 2022
Gregory G. Grecco, Jui Yen Huang, Braulio Muñoz, Emma H. Doud, Caliel D. Hines, Yong Gao, Brooke Rodriguez, Amber L. Mosley, Hui-Chen Lu, Brady K. Atwood
digestion at 35°C (
1:100
protease:substrate ratio,
Mass Spectrometry
grade,
Promega Corporation
, Cat No:
V5072
.) (17, 18). Peptide
Purification
and
Labeling
Digestion was halted by addition of 0.3% v/v trifluoroacetic
(83)
FBXO47 is essential for preventing the synaptonemal complex from premature disassembly in mouse male meiosis
iScience
April 15, 2022
Tanno N, Takemoto K, Takada-Horisawa Y, Shimada R, Fujimura S, Tani N, Takeda N, Araki K, Ishiguro KI.
(24x9.3mg)",
Thermo Fisher Scientific
,Cat#
90034
"
Trypsin
/Lys-C Mix, Mass Spec Grad (5x20μg)",
Promega
,Cat#
V5073
"cOmplete, EDTA-free",Roche,Cat# 4 693 132 RIPA buffer,Thermo,Cat# 89900 collagenase,Sigma-Aldrich,Cat#
(84)
Parallel kinase pathways stimulate actin polymerization at depolarized mitochondria
Current biology : CB
April 11, 2022
Fung TS, Chakrabarti R, Kollasser J, Rottner K, Stradal TEB, Kage F, Higgs HN.
alternative,Calbiochem,Cat#492016
Laemmli sample buffer
,
BioRad
,Cat#161-0747 "
Trypsin/Lys-C Mix, Mass Spec Grade
",
Promega
,Cat#
V5071
BAPTA-AM,Calbiochem,Cat#196419 CGP37157,Sigma-Aldrich,Cat#C8874 Go6976,Calbiochem,Cat#
365250
EGTA
,
Sigma-Aldrich
,Cat#E3889
Thapsigargin
,Calbiochem,Cat#586005 CK666,
Sigma-Aldrich
,Cat#SML0006
STO-609
,Calbiochem,Cat#570250 ML141,Sigma-Aldrich,Cat#SML0407 CCCP,Sigma-Aldrich,Cat#C2759 Ionomycin,
Sigma-Aldrich
,Cat#
I0634
Metformin,Sigma-Aldrich,Cat#PHR1084 DMSO,
Invitrogen
,Cat#
D12345
Critical
(85)
Prenatal Opioid Exposure Impairs Endocannabinoid and Glutamate Transmission in the Dorsal Striatum
eneuro
April 8, 2022
Gregory G. Grecco, Braulio Muñoz, Gonzalo Viana Di Prisco, Emma H. Doud, Brandon M. Fritz, Danielle Maulucci, Yong Gao, Amber L. Mosley, Anthony J. Baucum, Brady K. Atwood
protein digestion
to derive peptides (
1:100
protease/substrate ratio,
mass spectrometry
grade; catalog #
V5072
,
Promega
; Levasseur et al., 2019; Li et al., 2020). Peptide cleanup and tandem mass tag isobaric
labeling
Digestions
(86)
Dysregulated RNA polyadenylation contributes to metabolic impairment in non-alcoholic fatty liver disease
Nucleic acids research
April 8, 2022
Jobbins AM, Haberman N, Artigas N, Amourda C, Paterson HAB, Yu S, Blackford SJI, Montoya A, Dore M, Wang YF,
et al
.
for recovery of residual beads. Samples were digested for 5 h with 1.5 μg of LysC/Trypsin (
Promega
,
V5071
) at 37°C with shaking. Bead slurry was further diluted to approximately 1 M urea by addition of 20 mM
(87)
Integrative analysis of non-small cell lung cancer patient-derived xenografts identifies distinct proteotypes associated with patient outcomes
Nature Communications
April 5, 2022
Shideh Mirhadi, Shirley Tam, Quan Li, Nadeem Moghal, Nhu-An Pham, Jiefei Tong, Brian J. Golbourn, Jonathan R. Krieger, Paul Taylor, Ming Li,
et al.
precipitation and digested overnight at 37 °C with 1 μg trypsin/Lys-C mixture (
Promega
, Catalog No:
V5073
)97. TMT labeling of peptides After overnight digestion, peptide concentration was measured using nanodrop
(88)
Analysis of RNA-containing compartments by hybridization and proximity labeling in cultured human cells
STAR protocols
March 18, 2022
Yap K, Chung TH, Makeyev EV.
streptavidin C1 magnetic beads,
Thermo Fisher Scientific
,Cat#:
11205D
Trypsin
/ Lys-C Mix,
Promega
,Cat#:
V5073
Ammonium bicarbonate,Sigma Aldrich,Cat#: 09830 NaOH,Sigma Aldrich,Cat#: S8045 NaCl,Sigma Aldrich,Cat#:
(89)
A stress-reduced passaging technique improves the viability of human pluripotent cells
Cell Rep Methods
February 28, 2022
Takahashi K, Okubo C, Nakamura M, Iwasaki M, Kawahara Y, Tabata T, Miyamoto Y, Woltjen K, Yamanaka S.
lauroyl sarcosinate (SLS),WAKO,Cat# 192-10382 "
Trypsin / Lys-C Mix, Mass Spec Grade
",
Promega
,Cat#
V5072
Ethyl acetate,WAKO,Cat# 051-00356 Acetonitrile,WAKO,Cat# 018-19853 Acetic acid,WAKO,Cat# 018-20061 Formic
(90)
Internalization and trafficking of CSPG-bound recombinant VAR2CSA lectins in cancer cells
Scientific Reports
February 23, 2022
Chris Kedong Wang et al.
and beads resuspended in 50 µl of 50 mM HEPES, pH 8 and 0.5 µg trypsin/LysC was added (
Promega
, Cat#
V5071
) and incubated overnight at 37 °C in a ThermoMixer with mixing at 1200 rpm. Beads were removed magnetically,
(91)
Proteomic analysis of archival breast cancer clinical specimens identifies biological subtypes with distinct survival outcomes
Nature communications
February 16, 2022
Asleh K, Negri GL, Spencer Miko SE, Colborne S, Hughes CS, Wang XQ, Gao D, Gilks CB, Chia SKL, Nielsen TO,
et al
.
resuspended in 100 μL of 200 mM
HEPES
pH 8 (
Sigma
, cat# H3375) containing trypsin/Lys-C mix (
Promega
, cat#
V5071
) at a ratio of
1:50
(μg/μg) protein to enzyme concentration and incubated for at least 14-h (overnight)
(92)
Extracellular vesicles: Major actors of heterogeneity in tau spreading among human tauopathies
Molecular therapy : the journal of the American Society of Gene Therapy
February 2, 2022
Leroux E, Perbet R, Caillierez R, Richetin K, Lieger S, Espourteille J, Bouillet T, Bégard S, Danis C, Loyens A,
et al
.
in the dark. Samples were adjusted to 3 mM EDTA and digested with 0.5 μg Trypsin/LysC mix (
Promega
#
V5073
) for 1h at 37°C, followed by a second 1h digestion with a second and identical aliquot of proteases.
(93)
Association of cardiometabolic microRNAs with COVID-19 severity and mortality
Cardiovascular Research
January 29, 2022
Clemens Gutmann et al.
serum was denatured, reduced, and alkylated before enzymatic digestion with Trypsin/LysC (
Promega
,
V5072
). Digested peptide solutions were acidified and cleaned up using a Bravo AssayMAP Liquid Handling Platform
(94)
Hybridization-proximity labeling reveals spatially ordered interactions of nuclear RNA compartments
Molecular Cell
January 20, 2022
Karen Yap, Tek Hong Chung, Eugene V. Makeyev
09830 Water for
chromatography
(LC-MS Grade),Merck,Cat#: 1153331000 Trypsin / Lys-C Mix,
Promega
,Cat#:
V5073
,, Critical commercial assays,Critical commercial assays,Critical commercial assays ,,
Pierce BCA Protein Assay kit
(95)
Comparative Phosphoproteomics of Neuro-2a Cells under Insulin Resistance Reveals New Molecular Signatures of Alzheimer's Disease
International journal of molecular sciences
January 17, 2022
Kim D, Jo YS, Jo HS, Bae S, Kwon YW, Oh YS, Yoon JH.
the dark. After the
bicinchoninic acid (BCA) protein assay
, a
1:50
ratio of trypsin Lys-C mixture (
V5073
;
Promega
) was added to 100 µg of protein for 4 h at 50 °C. After
centrifugation
at 16,000× g for 10
(96)
Tumor collection/processing under physioxia uncovers highly relevant signaling networks and drug sensitivity
Science advances
January 14, 2022
Kumar B, Adebayo AK, Prasad M, Capitano ML, Wang R, Bhat-Nakshatri P, Anjanappa M, Simpson E, Chen D, Liu Y,
et al
.
M and digested overnight with trypsin/Lys-C mix
mass spectrometry
(
1:100
protease:substrate ratio;
V5072
,
Promega
). For phosphopeptide analysis, 360 μg of protein from
EpCAM
+ PyMT
tumor
cells under 3% O2 and
(97)
Quantification of the trans-synaptic partners neurexin-neuroligin in CSF of neurodegenerative diseases by parallel reaction monitoring mass spectrometry
eBioMedicine
January 3, 2022
Elena Camporesi et al.
iodoacetamide in 50 mM ABC and shaken for 30 min in the dark. Next, 25 µL of trypsin/Lys-C (
Promega
, cat.#
V5073
), corresponding to 0.5 µg per sample, were added to every sample and digestion was performed at 37°C
(98)
Effect of Trastuzumab–HER2 Complex Formation on Stress-Induced Modifications in the CDRs of Trastuzumab
Frontiers in Chemistry
January 3, 2022
Baubek Spanov, Victoria Aboagye, Oladapo Olaleye, Natalia Govorukhina, Nico C. van de Merbel, Rainer Bischoff
from Thermo Fisher Scientific (Waltham, MA, United States).
Trypsin/Lys-C mix, Mass Spec Grade
(cat #
V5073
), was obtained from
Promega
(Madison, WI, United States). Difluoroacetic acid (DFA, cat #
162120025
)
(99)
AT-hook DNA-binding motif-containing protein one knockdown downregulates EWS-FLI1 transcriptional activity in Ewing's sarcoma cells
PloS one
January 1, 2022
Kitagawa T, Kobayashi D, Baron B, Okita H, Miyamoto T, Takai R, Paudel D, Ohta T, Asaoka Y, Tokunaga M,
et al
.
37°C for 1h in the dark. The beads were added with 2.5 μl of 200 ng/μl
Trypsin
/Lys-C Mix (Cat. No.
V5073
,
Promega
) at 37°C with shaking overnight. The supernatant was collected by centrifuge, collected again
(100)
Multiomics analysis reveals the mechanical stress-dependent changes in trabecular meshwork cytoskeletal-extracellular matrix interactions
Frontiers in cell and developmental biology
January 1, 2022
Soundararajan A, Wang T, Sundararajan R, Wijeratne A, Mosley A, Harvey FC, Bhattacharya S, Pattabiraman PP.
at 37°C (
1:100
protease: substrate ratio,
mass spectrometry
grade,
Promega Corporation
, Madison, WI,
V5072
). Peptide
purification
and
labeling
Digestions were acidified with trifluoracetic acid (TFA, 0.5%
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Trypsin/Lys-C Mix, Mass Spec Grade from Promega
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